Recently, trends in Plant Science published a review paper entitled "mass specialty untangles plant membrane protein signaling networks" invited by Professor Chen Yanmei, Key Laboratory of plant physiology and biochemistry, China Agricultural University. Since its invention, mass spectrometry has won many Nobel prizes in chemistry and physiology. In recent years, it has been widely used in plant biology research. This review summarizes the application of mass spectrometry in the study of cell membrane protein, especially the function of kinase, membrane protein phosphorylation and protein interaction, and discusses the application of these techniques in plant biology, and puts forward effective analytical methods. As an important component of material transport, energy conversion and signal sensing between plants and their environment, cytoplasmic membrane plays an important role in the life process of plant growth and development and response to adversity. Membrane proteins are the main carriers of plasma membrane function. According to their distribution and location on plasma membrane, they are mainly divided into three categories: outer membrane proteins, integrated membrane proteins and lipid anchored proteins. The plasma membrane is rich in receptor kinases, channel proteins and enzymes, which play a key role in plant physiological and biochemical processes. However, it is very difficult to analyze and detect membrane protein because of its high complexity, low relative content and strong hydrophobicity. On the basis of these research areas in recent years, this review focuses on the application of mass spectrometry in the study of plasma membrane signal network, including the full spectrum analysis of plasma membrane protein, plasma membrane protein phosphorylation, protein kinase substrate interaction, lipid raft microdomain protein and membrane protein interaction.
It is very difficult to detect membrane protein and its phosphorylation, and it is easy to be interfered by background noise. In this review, the technical challenges and solutions for the study and analysis of membrane proteins and phosphorylation modification of membrane proteins are described. In addition, the enrichment and purification methods of protein phosphorylation, as well as the problems needing attention in practical application were discussed.
Fig. signal network of plasma membrane proteins in plant cells.
In addition, the application of mass spectrometry in the study of proteomics and phosphorylation of lipid rafts was reviewed. In addition to the summary of qualitative and quantitative analysis methods, this paper focuses on the method and application of mass spectrometry in the detection of kinase substrate. On the basis of these, the research progress of mass spectrometry in the field of membrane protein interaction is introduced in detail. At the same time, how to use mass spectrometry to study protein phosphorylation and protein interaction is also discussed, which provides a new idea for the research of plant plasma membrane protein.
As the first unit of this paper, Professor Chen Yanmei and Professor wolfram Weckwerth from the centre of metabolomics of the University of Vienna in Austria are co authors. This paper is supported by NSFC.
Original link:
https://www.sciencedirect.com/science/article/pii/S1360138520301138
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