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On April 17, 2020, molecular plant published a research paper entitled cold induced cbf-pif3 interaction enhancements freeing tolerance by stabilizing the phyB thermosensor in Arabidopsis from Professor Yang Shuhua's research group of China Agricultural University. The paper revealed the mechanism of cbf-pif3 interaction to enhance plant low temperature tolerance by stabilizing phyB.
The strategies of growth inhibition and cold acclimation can help plants resist the low temperature stress which has adverse effects on growth and survival. Phytochrome B (phyB) regulates plant growth by sensing light and ambient temperature signals. However, the mechanism of phyB mediated plant response to low temperature stress is still unclear.
This study showed that the important transcription factors mediate the mechanism of cold acclimation, i.e. C-repeat binding factors (CBFS) interact with phytochrome-interacting Factor3 (PIF3) under cold stress, thus weakening the co degradation of pif3-phyb. Low temperature stable phyB can regulate the expression of stress response genes and growth related genes, and regulate the role of cold resistance in the downstream of CBFS. PhyB mutants showed a frost sensitive phenotype, while the transgenic plants overexpressing phyB showed stronger frost resistance. Further analysis showed that pif1, pif4 and pif5 proteins, which negatively regulated plant cold tolerance, were degraded in a light pigment dependent manner under low temperature stress.
In conclusion, this study revealed that cbfs-pif3-phyb is an important regulatory module of plant response to cold stress. The interaction of CBFS and PIF3 integrated the low temperature signal and phyB mediated light signal, which clarified the important role of phyB in plant response to low temperature stress.
The role of cbf-pif3-phyb module in the regulation of Arabidopsis cold tolerance
Attachment:
Abstract
Growth inhibition and cold acclimation strategies help plants withstand cold stress which adversely affects growth and survival. PHYTOCHROME B (phyB) regulates plant growth through perceiving both light and ambient temperature signals. However, the mechanism by which phyB mediates the plant response to cold stress remains elusive. Here, we show that the key transcription factors mediating cold acclimation, C-REPEAT BINDING FACTORs (CBFs), interact with PHYTOCHROME-INTERACTING FACTOR3 (PIF3) under cold stress, thus attenuating the mutually assured destruction of PIF3-phyB. Cold-stabilized phyB acts downstream of CBFs to positively regulate freezing tolerance by modulating the expression of stress-responsive and growth-related genes. Consistently, phyB mutants exhibited a freezing-sensitive phenotype, whereas phyB-overexpression transgenic plants displayed enhanced freezing tolerance. Further analysis showed that PIF1, PIF4, and PIF5 proteins, all of which negatively regulate plant freezing tolerance, were destabilized by cold stress in a phytochrome-dependent manner. This study thus uncovers that CBFs-PIF3-phyB serves as an important regulatory module for regulating plant response to cold stress.
Article link: https://doi.org/10.1016/j.molp.2020.04.006
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