Introduction: "innovation, change, breaking the situation", entering a new era, in order to further deepen the "three aspects", promote the academic exchange of animal nutrition, and help the growth of young talents, "summer peace Youth Award" came into being. Young scholars from 27 member units under the Key Laboratory of animal nutrition and feed subject group of the Ministry of agriculture and rural areas will present their recent years to the industry one by one Major research results. Their efforts, efforts and gains will be the perfect interpretation of the continuous development and innovation of animal nutrition people in the new era!
Dr. He Pingli
Feed safety and biological potency of the Ministry of agriculture and rural areas
Young scholars recommended by key laboratories
Personal profile
Female, China Agricultural University, School of animal science and technology, associate researcher, doctoral supervisor education experience:
2012 / 11-2013 / 10, visiting scholar, Department of chemistry and biochemistry, University of Maryland; 1999 / 09-2004 / 07, Department of chemistry, Beijing Normal University; 1999 / 09-1999 / 07, Department of chemistry, Hunan Normal University, bachelor's research experience:
From January 2007 to now, associate researcher, School of animal science and technology, China Agricultural University; from July 2004 to December 2006, assistant researcher, School of animal science and technology, China Agricultural University. The main research fields are drug residue and anti nutritional factor detection, nutritional metabolism and proteomic analysis. A series of rapid detection and instrument confirmation methods for veterinary drugs, antinutritional factors and toxins were developed. Presided over national natural fund projects (3 projects), key research and development subprojects (2 projects), "973" plan, science and technology of public welfare industry and other national level projects. He has published nearly 40 SCI papers, 8 patents, won the first prize of science and technology progress of the Ministry of education, the second prize of national science and technology progress award, the Shennong China Agricultural Science and technology award, and won the 2016 Dabei agricultural young scholars award. Summary of achievements
(1) Using the advantages of immunology, analytical chemistry, molecular biology, nanoscience and other interdisciplinary disciplines, a variety of new technologies and products for simultaneous and rapid detection of veterinary drug residues, mycotoxins and other new products have been developed, providing a strong technical guarantee for the safety monitoring of feed and animal products.
The relevant work has been published by the first author or corresponding author in internationally renowned analytical journals. For example, 32 kinds of β - adrenergic agonist drugs synchronous detection technology based on nano gold has been developed, which can be observed by naked eyes without any instrument, and the detection time is 5 minutes. The results are concerned by chemical and Engineering News, a news column of American Chemical Society. It is evaluated that the establishment of this method has opened up a new idea for food safety detection (Analytical Chemistry, 2011, 83 (18)), 6988-6995, if 6.35; patent zl201410509411.3); a total of 26 test strips for rapid detection of drug residues in β - receptor agonists, tetracyclines and sulfonamides have been developed. The sample does not need any pretreatment, no instrument, 10 minutes results and the sensitivity is 10 times higher than the existing products (analytica chimica Acta 927 (2016) 64-71, if 5.41); developed three kinds of main mycotoxins (aflatoxin B1, zearalenone, vomit toxin) synchronous and rapid detection technology based on fluorescent quantum dots, without large-scale instrument, which can be measured by naked eye, detection time is 15 minutes, interference is small, accuracy and sensitivity of the method reach the level of liquid chromatography-mass spectrometry (microchimica Acta, 2019, DOI: 10.1007/s00604-019-3879-6, IF 5.48 ）。
(2) This paper established the detection technology system of soybean anti nutritional factors, solved the key technical problems of rapid and accurate detection, and provided technical means for the efficient utilization and quality control of soybean protein source in feed. For example, the standard products of three kinds of protein anti nutritional factors were prepared, and the single and polyclonal antibodies of four kinds of soybean antigen proteins were obtained. The trypsin inhibitor, soybean antigen protein and other soybean anti nutritional factors in soybean were detected by enzyme-linked immunosorbent assay (analytica chimica Acta, 2012, 734, 62-68, if 5.41, Patent zl201010577271.5, etc.), relevant achievements won the second prize of 2015 National Science and Technology Progress Award (ranking the fourth), one of 2016-2017 Shennong China Agricultural Innovation Team Award (ranking the sixth), and for the first time at home and abroad developed a monoclonal antibody ELISA kit for soybean globulin, β - paraglobulin and trypsin inhibitor, which has been industrialized, and began to be applied in feed industry It creates good social and economic benefits.
(3) The simultaneous screening and quantitative detection of vitamins, amino acids, β - adrenergic agonists, antibiotics and pigments in feed were established by high-end instrumental analysis techniques such as liquid chromatography tandem mass spectrometry and gas chromatography tandem mass spectrometry. For example, we created a broad-spectrum screening technology for β - receptor agonists based on high-resolution mass spectrometry, constructed a database of hundreds of veterinary drugs and banned substances, and realized the rapid confirmation and screening of unknown drugs (Food Chemistry, 192, 2016, 188-196, if 5.40; special zl201410563021.4). This paper was listed as a highly cited paper by web of science.
Representative papers of achievements
1、Fluorometric lateral flow immunoassay for simultaneous determination of three mycotoxins (aflatoxin B1, zearalenone and deoxynivalenol) using quantum dot microbeads.
Abstract: in this paper, a rapid and synchronous method for the detection of three mycotoxins (aflatoxin B1 (AFB1), zearalenone (Zen), vomit toxin (Don)) was introduced. The method is based on CdSe / SiO2 quantum dot microspheres (QBs) with an average diameter of 106 nm. These QDs have strong red emission ability and enhance the sensitivity. It can bind to mAbs against AFB1, Zen and don respectively, and can detect AFB1, Zen and don in grain samples respectively. The fluorescence signal intensity of the detection line has a negative correlation with the content of the substance to be measured, and the quantitative analysis of three mycotoxins can be realized within 15 minutes. The detection limits of AFB1, Zen and don were 10 PG ML? 1, 80 PG ML? 1 and 500 PG ML? 1, respectively. The results showed that the average recovery of the method was 85.5 ~ 119.0%, and the relative standard deviation was less than 16.4%. The results of LC-MS / MS analysis are in good agreement with that of LC-MS / MS analysis.
Key words: immune test strip, antibody, rapid detection, feed, mycotoxin research subject: national key research and development plan "research and development of key food safety technology" project "Research on migration and transformation mechanism of key hazards in main livestock and poultry products and safety control mechanism" (subject No.: 2017yfc1600300). 2、Broad screening and identification of β-agonists in feed and animal body fluid and tissues using ultra-high performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry combined with spectra library search.
Abstract: in this study, we used the technology of ultra-high performance liquid chromatography and high resolution mass spectrometry, using trance In order to establish a broad-spectrum screening method for β - adrenergic agonists in feed, animal body fluid and tissue samples, a spectrum library of 32 β - adrenergic agonists was constructed by finder software, and the actual samples were purified by the mixed cation exchange solid-phase extraction method. 10 blank addition samples, 108 test animal samples and 130 unknown samples collected from the market were used to verify the applicability of the library. The results show that the search accuracy of the library is 98%, and there is no false negative result. In addition, for drugs without standards, only three parameters of mother ion accurate mass to charge ratio, isotope distribution and secondary fragment ion can be used to search the spectrum library, which greatly reduces the difficulty of screening and confirmation of β - receptor agonist drugs in feed and livestock products. Key words: β - receptor agonist drugs, livestock and poultry products, identification, rapid liquid chromatography-high resolution mass spectrometry, spectral library search research subject: research and demonstration of ruminant β - receptor agonist metabolic residue rule and monitoring key technology (Project No.: 201203088), a special scientific research project of public welfare industry (Agriculture). 3、A sensitive and semi-quantitative method for determination of multidrug residues in animal body fluids using multiplex dipstick immunoassay.
Abstract: This study developed a microstrip based gold gel based strip test for simultaneous detection of 26 kinds of drugs including beta agonist, sulfonamides and tetracycline in animal urine and serum. The multi-component simultaneous detection of immune test paper is based on an indirect competition method, that is, three test lines (different antigens) and one control line (Goat anti mouse IgG) are respectively located on four lines of the test paper. Labelled antibodies were freeze-dried in gel. The sample does not need pretreatment and can be analyzed directly within 10 minutes. The linear relationship between the concentration of drug residues and the absorbance of gold nanoparticles was used as the basis for quantitative determination. The results showed that the detection threshold of clenbuterol hydrochloride was 0.3-0.45 ng ml-1, sulfadiazine 3-4 ng ml-1 and tetracycline 4.5-6 ng ml-1, respectively. The recoveries of clenbuterol hydrochloride, sulfadiazine and tetracycline were 78.4% - 112.6%, and the RSD were all below 11.2%. The analysis of animal samples shows that the accuracy of the method proposed in this paper is consistent with that of LC-MS / MS. The percentage of error results is less than or equal to 5%. Therefore, the multi-component strip method is cheap, easy to use, and suitable for rapid and comprehensive screening of three categories of 26 drug residues in animal body fluids. Key words: multi reaction monitoring test strip, β - receptor agonist drugs, tetracycline drugs, sulfonamides drugs, research subjects of animal body fluids: general program of National Natural Science Foundation of China "Application Research of homogeneous visualization technology based on nano gold and quantum dots in multi-component simultaneous detection" (Project No.: 31472126) ". Invention patent
1. Invention patent + detection method and kit of B-receptor agonists in animal body fluids + he Pingli, Wang Xian, Li Tingting, Li Qin + China Agricultural University
2. Invention patent + a method of broad-spectrum identification of B-receptor agonist drugs + he Pingli, Li Tingting, Cao Jingjing, Li Qin + China Agricultural University
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Dr. Cao Zhijun, Professor of China Agricultural University
Recommended young scholars of "Yihe" Youth Award 2020 Dr. Wang WENCE, associate professor of South China Agricultural University, recommended young scholars of "Yihe" Youth Award 2020 Dr. Chu Beibei, Professor of Henan Agricultural University
Dr. Wang Min, researcher of subtropical Research Institute of Chinese Academy of Sciences, and Dr. Yu Qinghua, Professor of Nanjing Agricultural University
Recommended young scholars of "Yihe" Youth Award 2020 Dr. Che Lianqiang, Professor of Sichuan Agricultural University, recommended young scholars of "Yihe" Youth Award 2020 Dr. Chen Liang, Beijing Institute of animal husbandry and veterinary medicine, Chinese Academy of Sciences
Dr. Wang Xinxia, associate professor, School of animal science, Zhejiang University
Dr. Jiang Weidan, Professor of Sichuan Agricultural University
Dr. Cheng Yanfen, Professor of Nanjing Agricultural University
Dr. Wang Songbo, Professor of South China Agricultural University
Dr. Zhou Zhenming, associate professor, School of animal science and technology, China Agricultural University