Recently, plant cell online published a research paper titled "spatial restriction of fusa3 expression by class I BPCS promotions ovule development and coordinates EMERYO and endosperm growth" jointly by Wu Jian team of School of horticulture, China Agricultural University and Sonia Gazzarrini, Department of biology, University of Toronto, Canada. This study revealed the molecular mechanism that BPCS transcription factors strictly regulate the temporal and spatial expression of fusca3 (Fus3), promote ovule development and maintain the synchronous development of embryo and endosperm. This study revealed the new function of Fus3 gene in regulating ovule development and elucidated the regulatory mechanism and role of fusca3 expression in embryo and inhibition in endosperm.
Figure BPC1 and 2 Negatively Regulate FUS3 Expression in Reproductive Organs and Seeds.
As a main food source, seeds provide about 70% energy for human and animals. Therefore, plant fertility is a very important economic character in crop breeding. The process of plant growth and development is precisely controlled by the genetic material in vivo. Among them, B3 family is involved in the process of seed development and dormancy. Previous studies by the Gazzarrini team found that knockout of Fus3 gene can lead to ovule and seed abortion (Chan et al., J exp BOT 2017). Overexpression of Fus3 gene (proml1: Fus3 EGFP) resulted in abnormal flower development and seed abortion (Gazzarrini et al., devel cell 2004).
The team further clarified the molecular mechanism of Fus3 regulating ovule and seed development. This study comprehensively analyzed the expression pattern of Fus3 in reproductive organs, and found that Fus3 was specifically expressed in the chalaza and funiculus of mature ovules, and in the chalaza, embryo, fruit stalk and seed coat of seeds after fertilization. It was found that I members of BPC subfamily can directly bind 5'UTR of Fus3 and GA / CT n elements of the first exon and intron. In addition, BPC subfamily I members can interact with PRC2 family members. This study elucidated that BPCS inhibited the expression of Fus3 in integument by recruiting PRC2 complex, and maintained the normal development of integument and embryo sac; elaborated the molecular mechanism that Fus3 was inhibited in endosperm due to the expression of MEA and fis2 members of fis-prc2 complex only in endosperm, so BPCS could not recruit fis-prc2 complex to inhibit the expression of Fus3 in embryo. It was also found that the ectopic expression of Fus3 in endosperm would lead to the abnormal development of endosperm and the delay or deformity of embryo development, which could eventually lead to seed abortion.
After the research was published online, it received extensive attention from international peers. Dr. Philip carlla, Sainsbury laboratory, Cambridge University, wrote a review article in plant cell.
The research was carried out by Wu Jian Laboratory of horticultural College of China Agricultural University and Sonia Gazzarrini Laboratory of Department of biology, University of Toronto, Canada. Dr. Wu Jian is the co first author and Dr. Sonia Gazzarrini is the corresponding author. Li Jingru, China Agricultural University, Deka Mohamed, Sebastian dowanik, Rosanna Petrella, Veronica gregisc and Wu Lin of Chongqing University of Arts and Sciences participated in the research. The research was supported by the National Natural Science Foundation of China, China Postdoctoral Science Foundation and Canadian natural science and engineering foundation. Link: http://www.plantcell.org/content/early/2020/04/07/tpc.19.00764 comment articles: http://www.plantcell.org/content/early/2020/04/17/tpc.20.00295 plant science frontier official account is the most powerful official account in the field of Botany. In order to promote the communication between disciplines, we have created 100 vertical subdivision discussions, covering 20000 active subjects directly. Users, here you can quickly ask professional questions from peers and get answers. At present, there are discussion groups in the following fields:
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